Small RNA interference inhibition of ubiquitin-specific protease 14 gene expression in intraphepatic cholangiocarcinoma primary cells.
บทคัดย่อ :
Abstract. Studies on genomic instabilities of intrahepatic cholangiocarcinoma
(ICC) from Thai patients have shown 52% variations in a specific region of
ubiquitin-specific protease 14 gene (USP14) located on 18p11.32. Inhibition of
USP14 expression in three different primary cells cultured from resected ICC
tissues by transfecting with double-strand RNA interference targeting exon 16
region of USP14 mRNA resulted in 6-50% reduction in mRNA levels, with cells
with low USP14 expression being more affected. These findings lend support to
a potential in developing strategies against USP14 in ICC.
Keywords: intrahepatic cholangiocarcinoma, primary cell culture, RNAi,
ubiquitin-specific protease 14
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